Preservation of Influenza Virus Infectivity by Lyophilization

The Preservation of Yeast Cultures by Lyophilization.

Since the pioneer work of Rogers (1914) on drying cultures from the frozen state and the wide application of this procedure to bacteria, the lyophil process has been shown to be a successful method for the preservation of yeast cultures in a viable state over extended periods (Elser et al., 1935; Wickerham and Andreasen, 1942; Wickerham and Flickinger, 1946). The fundamental value of this metho...

The Mouse Infectivity Titration of Influenza Virus

A study has been made to establish the statistical significance of results obtained in mouse infectivity titrations of influenza virus. Five titrations, each composed of five replicas, were carried out and 50 per cent end points were calculated for each titration. Three criteria for evaluating the end points were employed, namely, the presence or absence of pulmonary lesions, the occurrence of ...

Preservation of rhizobia by lyophilization with trehalose(1)

– The objective of this study was to determine if trehalose can effectively enhance the viability and storage lifetime of Rhizobium tropici and Rhizobium etli after lyophilization as compared to the traditional protective combination of sucrose and peptone. Two strains of bean-nodulating rhizobia were effectively preserved in the lyophilized state by 100 mM trehalose, and survived for at least ...

Preservation of Serratia marcescens by high-vacuum lyophilization.

Water-washed Serratia marcescens (ATCC strain 14041) cells were lyophilized in an all-glass system capable of evacuation to pressures of less than 5 x 10(-6) torr. Lyophilization at the lowest pressures resulted in 50 to 65% survival for unstabilized washed organisms compared with 10 to 20% when the cells were lyophilized at pressures of about 2.5 x 10(-2) torr. At the latter pressures, 45 to 6...

Title Individual Correlates of Infectivity of Influenza A Virus Infections